Effect of Acetonitrile in Eluent (2)

When analyzing organic acids using RSpak KC-811, the elution time of organic acids with strong hydrophobicity can be shortened by adding 10% acetonitrile to the eluent.


Sample :
1. Succinic acid
2. Lactic acid
3. Formic acid
4. Acetic acid
5. Propionic acid
6. Isobutyric acid
7. n-Butyric acid
8. Isovaleric acid
9. n-Valeric acid

Columns      : Shodex RSpak KC-LG (8.0mmID*50mm) + KC-811 (8.0mmID*300mm) x 2
Reagent      : 10-fold diluted ST3-R(for post-column method)
Flow rate    : (Eluent); 1.0mL/min, (Reagant); 0.5mL/min 
Detector     : VIS(430nm)
Column temp. : 47deg-C