Methylxantines (2)

Theobromine, theophylline, caffeine were separated by RSpak DE-413 (4.6mmID x 150mm) and DE-213 (2.0mmID x 150mm). As DE-213 is a semi-micro type column, it can be also used for LC/MS.


Sample :
1. Theobromine
2. Theophylline
3. Caffeine

              (Left)
Column       : Shodex RSpak DE-413(4.6mmID*150mm)   
Eluent       : H2O/CH3CN=75/25
Flow rate    : 0.5mL/min
Detector     : UV(254nm)
Column temp. : 30deg-C

 (Right)
: Shodex RSpak DE-213(2.0mmID*150mm)
: H2O/CH3CN=75/25
: 0.1mL/min
: UV(254nm)
: 30deg-C