Local Anesthetics (2)

Local anesthetics were analyzed using RSpak DE-413 (a column for reversed phase chromataography) with alkaline eluent.
Please refer to Analysis of Short Amines without Using Ion Pair Reagent.


Sample : 10micro-L
1. Benzocaine 20micro-g/mL
2. Lidocaine 100micro-g/mL
3. Tetracaine100micro-g/mL

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : 20mM Sodium phosphate buffer(pH9.0)/CH3CN=50/50
Flow rate    : 0.8mL/min
Detector     : UV(254nm)
Column temp. : 40deg-C