LC/MS Analysis of Drugs in Control Serum (ODP2 HP)

LC/MS analysis of drugs in control serum after removing proteins was done by polymer-based column ODP2 HP. Each drug has peak areas between 82 – 111 % (peak areas without BSA are set as 100 %), which means a good separation without receiving ion suppression from protein.


Sample : drugs 500ng/mL each in Control serum, 5micro-L
Lidocaine
Chlorpheniramine
Propranolol
Diphenhydramine
Bupivacaine
Verapamil
Imipramine

Column       : Shodex ODP2 HP-2B (2.0mmID*50mm)
Eluent       : 10mM Ammonium acetate aq./CH3CN=70/30
Flow rate    : 0.2mL/min
Detector     : UV(280nm), ESI-MS (SIM Positive)
Column temp. : 40deg-C