Milk

Anions in milk were analyzed using IC I-524A. Milk contains a considerable amount of proteins and fats, and therefore should be pretreated. 100-fold dilution with pure water was made and then treated by an solid phase extraction cartridge for reversed phase chromatography.


Sample : 100-fold diluted Milk, 50micro-L
2. HCO₃^-
3. Cl^-
6. NO₃^-
7. HPO₄^2-

Sample : Anion Standards, 50micro-L
1. F- 5mg/L
2. HCO₃^-30mg/L
3. Cl^-5mg/L
4. NO₂^- 5mg/L
5. Br^- 10mg/L
6. NO₃^- 10mg/L
7. HPO₄^2-10mg/L
8. SO₄^2- 10mg/L

Column       : Shodex IC I-524A (4.6mmID*100mm)
Eluent       : 1.5mM p-Hydoxybenzoic acid + 1.7mM N,N-Diethylethanolamine + 10% CH3OH
Flow rate    : 1.5mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40deg-C