Serum

The problems in the anaysis using IC YK-421 is that the analysis of very small amount of NH₄⁺ in a sample which contains large amount of Na⁺ is difficult. Addition of 1.5mM 18-Crown 6-ether in 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid eluent can solve the problem. Addition of crown 6-ether can adjust the elution volume of the cations. Especially, the elution volume of K⁺ is largely affected by the additon and elute after Mg²⁺.


Sample : Serum
1. Na⁺
2. NH₄⁺
3. Ca²⁺
4. Mg²⁺
5. K⁺

Column       : Shodex IC YK-421 (4.6mmID*125mm)
Eluent       : (left) 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid
               (right)5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid + 1.5mM 18-Crown 6-ether
Flow rate    : 1.0mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40deg-C