Tetraalkylammoniums (YK-421)

Trialkylammonium were separated using cation chromatography column, IC YK-421. By using the mobile phase used below (high ACN concentration), hydrophobic interaction is suppressed. Thus ioninc-interaction dominates, this makes shorter chain alkylammonium to be retained longer than longer ones.


Sample : 100mg/L each, 20micro-L
1. Tetrahexylammonium
2. Tetrapentylammonium
3. Tetrabutylammonium
4. Tetrapropylammonium
5. Tetraethylammonium
6. Tetramethylammonium

Column       : Shodex IC YK-421 (4.6mmID*125mm)
Eluent       : 2.5mM HNO3 aq./CH3CN=30/70
Flow rate    : 1.0mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40deg-C