Cobalt Ion by Chemiluminescence Detection

Highly sensitive analysis of cobalt ion is possible using IC T-521 and a chemiluminescence detector (CLD). When 500 micro-L of sample is directly injected, ng/L(=ppt) level detection is possible. Reagents should be prepared just before they are used. And, they should be kept cooling with ice water in a dark place.


Sample : Co²⁺

Column       : Shodex IC T-521 (4.6mmID*150mm)
Eluent       : 6mM Oxalic acid + 3mM Citric acid + 0.1mM NaN3(pH4.1)
Reagent      : (A); 1mM Luminol in 0.1M Borate buffer(pH11.0), (B); 1mM H2O2
Flow rate    : (Eluent); 1.0mL/min, (Reagent A); 0.5mL/min, (Reagent A); 1.5mL/min
Detector     : CLD
Column temp. : 40deg-C