Chelating Compounds (ODP-40)

Chelating compounds were analyzed with Asahipak ODP-40 4D (a column for reversed phase chromatography).

(Sample preparation)
The chelating agents were dissolved into 10mM sodium bicarbonate and 10mM sodium carbonate aqueous solution whose pH is adjusted to 11.1 by tetrabutyl ammonium hydroxide. After the adjustment of pH, the surplus amount of 0.01M iron chloride solution was added for making chelating compounds.



Sample : 100ppm each, 10micro-L
1. (2-Hydroxyethyl)ethylenediamine-N,N',N'-triacetic Acid, HEDTA
2. Ethylenediaminetetraacetic acid disodium salt, EDTA
3. Diethylenetriaminepentaacetic acid dianhydride, DTPA

Column       : Shodex Asahipak ODP-40 4D (4.6mmID*150mm)
Eluent       : 10mM Na2CO3 + 10mM NaHCO3 
               + 10mM Tetrabutylammonium Hydroxide (pH7.0 adjusted with 1M of HCl)
Flow rate    : 0.8mL/min
Detector     : UV(254nm)
Column temp. : 40deg-C