Elution Volume for Lipid and Fatty Acids (EV-2000 AC)

Elution volume for lipid and fatty acids were monitored by CLNpak EV-2000 AC. Lipid and fatty acids elute fast, thus by fractionating between acrinathrin and tricyclazol, most lipid and fatty acids can be removed.


Sample : 5mL
Beef tallow
Acrinathrin
Tricyclazole
Oleic acid
Palmitic acid
Linolenic acid
Linoleic acid

Column       : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
Eluent       : Acetone/Cyclohexane=1/4
Flow rate    : 5mL/min
Detector     : UV(254nm)
Column temp. : 40deg-C