Columns for GPC Clean-up of Phthalic Acid Esters: CLNpak PAE Series

Phthalic acids are suspected to be one of endocrine disruptors. Generally, troublesome sample-pretreatment is necessary to analyze pesticide residues and endocrine disruptors. Columns for GPC clean-up of phthalic acid esters, CLNpak PAE series were developed to simplify the sample-preteatment. As shown in left figure, lot-to-lot reproducibility of the columns is fairy good because the columns are packed with polymer-based materials. The specifications of the columns are shown below.

Type	Size(mm) IDxL	Maximum pressure	Packing material	In-column solvent	Usable eluent
CLNpak PAE-800 CLNpak PAE-2000 CLNpak PAE-G CLNpak PAE-800 AC CLNpak PAE-2000 AC CLNpak PAE-G AC	8.0 x 300 20.0 x 300 8.0 x 50 8.0 x 300 20.0 x 300 8.0 x 50	40MPa 5MPa - 4MPa> 5MPa -	Poly(vinylalcohol) gel	Acetonitrile Acetonitrile Acetonitrile Acetone Acetone Acetone	Aqueous solution (0 to 0.5M) Ethanol, Methanol, Acetone Acetonitrile, Propanol Ethyl acetate, THF, DMF Dioxane, Chloroform DMSO (0 to 50%)

The typical analysis conditions for CLNpak PAE sereis is as follows:

Type	Eluent	Flow rate	Maximum sample load
CLNpak PAE-800 CLNpak PAE-2000 CLNpak PAE-800 AC CLNpak PAE-2000 AC	Acetonitrile Acetonitrile Acetone Acetone	0.5mL/min 3.1 to 3.5mL/min 0.5mL/min 3.1 to 3.5mL/min	500micro-L (Water:10%) 3000micro-L (Water:10%) 500micro-L (Water:10%) 3000micro-L (Water:10%)