Titration Curve of IEC DEAE-825

IEC DEAE-825 and Asahipak ES-502N are packed with weak anion exchange resins bonded with diethylaminoethyl groups. The pKa of diethylaminoethyl groups is approximately 7.8, therefore DEAE-825 and ES-502N are generally recommended for the separation of acidic proteins and used most commonly for ion exchange chromatography of proteins. DEAE-420N packed with non-porous gel is suitable for fast analysis.