Oligopeptides (SB401-4E)
Oligopeptides were analyzed using
SB401-4E (a column for high perfonmance semi-micro aqueous SEC (GFC)).
Sample : 10micro-L
1.
Gly-Gly-Gly
2.
Gly-Gly
3.
Glycine
Column : Shodex SB401-4E (4.6mmID*250mm)
Eluent : 50mM CH3COONH4 aq./CH3CN=90/10
Flow rate : 0.2mL/min
Detector : UV(210nm) (small cell volume)
Column temp. : 25deg-C