Soysaponin in Soy Bean

Soysaponin in soy bean were analyzed using RSpak DS-613 (a column for reversed phase chromatography).


Sample :
1-14. Unknown
15. Soysaponin Bb
16-21. Unknown

Column       : Shodex RSpak DS-613 (6.0mmID*150mm)
Eluent       : CH3COOH/H2O/Isopropanol/CH3CN=0.1/63.4/4.2/32.3
Flow rate    : 1.0mL/min
Detector     : UV(205nm)
Column temp. : 25 to 27deg-C