Methionine-beta-Naphthylamide

Optical rotation of each peak can be monitored by using a combination of an optical rotation detector (ORD) and ORpak CDB-453 HQ (a column for chiral separation). A positive peak shows dextrorotation, while a negative peak shows levorotation. The analysis of methionine-beta-naphthylamide, an amino acid using a UV detector and an optical rotation detector is shown.


Sample : Methionine-beta-naphthylamide

Column       : Shodex ORpak CDB-453 HQ (4.6mmID*150mm)
Eluent       : [1.4% CH3COOH + 0.7% Triethylamine + 0.1M NaCl (pH3.9)]/CH3CN=80/20
Flow rate    : 0.2mL/min
Detector     : UV(300nm), ORD
Column temp. : 20deg-C