ATP Related Substances in Muscle of Sea Food

ATP related substances in muscle of sea food were analyzed using Asahipak GS-320 7G ( a multimode column ).
GS-320 7G is an old type of GS-320 HQ.

(A) Standards
(B) Sea bass just after death
(C) Ascidian after strage for one day at 2deg-C
(D) Spring lobster after strage for 12 hours at 20deg-C


Sample :
1. ATP
2. ADP
3. AMP
4. IMP
5. Inosine
6. Hypoxanthine

Column       : Shodex Asahipak GS-320 7G (7.5mmID*500mm)
Eluent       : 200mM Sodium phosphate buffer(pH2.9 to 3.0)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : Room temp.

Courtesy of Dr. Yamanaka, Tokyo Unversity of Fisheries and Dr. Matsumoto, Ochanomizu University