Mycotoxins = Aflatoxins

Aflatoxins

Aflatoxins were analyzed with Silica C18M 4E column after the sample pretreatment.

Sample : 5micro-g/L each, 20micro-L

	(TFA method)		(PR method)
1.	Derived Aflatoxin G₁	1.	Aflatoxin G₂
2.	Derived Aflatoxin B₁	2.	Aflatoxin G₁
3.	Aflatoxin G₂	3.	Aflatoxin B₂
4.	Aflatoxin B₂	4.	Aflatoxin B₁

(TFA method)		(PR method)
Column	: Shodex Silica C18M 4E (4.6mmID*250mm)	Column	: Shodex Silica C18M 4E (4.6mmID*250mm)
Eluent	: CH₃CN/CH₃OH/H₂O=10/30/60	Eluent	: CH₃OH/H₂O=40/60
Flow rate	: 1.0mL/min	Flow rate	: 0.7mL/min
Detector	: Fluorescence(Ex.365nm, Em.450nm)	Detector	: Fluorescence(Ex.365nm, Em.450nm)
Column temp.	: 40deg-C	Column temp.	: 40deg-C