Mycotoxins of Trichothecene Type

Deoxynivalenol(DON) and Nivalenol(NIV) in grains were analyzed with Silica C18M 4E after the sample pretreatment.


Sample : 20micro-L
(A) Standard solution (The amount of DON and NIV are 10ng each.)
(B) Sample solution of Wheat spiked with DON and NIV at 1micro-g/mL each
Nivalenol(NIV)
Deoxynivalenol(DON)

Column      : Shodex Silica C18M 4E (4.6mmID*250mm)
Eluent      : H2O/CH3OH/CH3CN=90/5/5
Flow rate   : 1.0mL/min
Detector    : UV(220nm)
Column temp.: 40deg-C