General Information on Packed Columns = Column Test Conditions

Size Exclusion Chromatography (SEC) Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
KW-802.5 to KW-804	(1)0.2% Ethylene glycol (2)30micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
KW402.5 to KW405	(1)0.05% Uridine(KW402.5-4F, KW403-4F) 0.02% Uridine(2)1micro-L (3)50mM Phosphate buffer + 0.3M NaCl(pH7.0) (4)0.35mL/min (5)Ambient (6)UV(280nm)
SB-802 HQ to SB-806M HQ	(1)1% Ethylene glycol(802 HQ), 0.5% Ethylene glycol(802.5 HQ to 806M HQ) (2)10micro-L(802 HQ), 15micro-L(802.5 HQ to 806M HQ) (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
SB-807 HQ	(1)0.5% Glucose (2)10micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
SB401 to SB404	(1)0.5% Ethylene glycol (2)5micro-L (3)H₂O (4)0.3mL/min (5)Ambient (6)RI
GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ	(1)1% Ethylene glycol (2)30micro-L (3)H₂O (4)0.6mL/min (5)30deg-C (6)RI
GS-320 4B GS-320 4D GS-320 2D	(1)0.5% Ethylene glycol (2)2micro-L(GS-320 4B, GS-320 4D), 1.5micro-L(GS-320 2D) (3)H₂O (4)0.25mL/min(GS-320 4B), 0.2mL/min(GS-320 4E), 0.05mL/min(GS-320 2D) (5)35deg-C (6)RI
GS-320 7E	(1)1% Ethylene glycol (2)25micro-L (3)H₂O (4)1.0mL/min (5)30deg-C (6)RI
GS-620 7G-P	(1)1% Ethylene glycol (2)50micro-L (3)H₂O (4)1.0mL/min (5)30deg-C (6)RI
GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ	(1)1% Ethylene glycol (2)30micro-L (3)H₂O (4)0.6mL/min (5)30deg-C (6)RI
GF-310 4B GF-310 4D GF-310 4E GF-310 2D	(1)0.5% Ethylene glycol (2)2micro-L(GF-310 4B, GF-310 4D), 3micro-L(GF-310 4E), 1micro-L(GF-310 2D) (3)H₂O (4)0.25mL/min(GF-310 4B), 0.2mL/min(GF-310 4D, GF-310 4E), 0.05mL/min(GF-310 2D) (5)35deg-C (6)RI
KF-801 to KF-807L	(1)0.1% Propylbenzene (2)20micro-L (3)THF (4)1.0mL/min (5)Ambient (6)UV(254nm)
K-801 to K-807L	(1)0.1% Propylbenzene (2)20micro-L (3)Chloroform (4)1.0mL/min (5)Ambient (6)UV(254nm)
KD-801 to KD-807	(1)1.0% Acetone (2)20micro-L (3)DMF (4)1.0mL/min (5)Ambient (6)RI
KF-601 to KF-607	(1)0.5% Propylbenzene(KF-601), 0.1% Dicyclohexyl phthalate(KF-602 to KF-607) (2)5micro-L (3)THF (4)0.5mL/min (5)Ambient (6)UV(254nm)
KF-401HQ to KF-406LHQ	(1)0.5% Propylbenzene(KF-401HQ), 0.1% Dicyclohexyl phthalate(KF-402HQ to KF-406LHQ) (2)5micro-L (3)THF (4)0.3mL/min (5)Ambient (6)UV(254nm)
HFIP-803 to HFIP-807	(1)5% Acetone (2)10micro-L (3)HFIP (4)1.0mL/min (5)Ambient (6)UV(280nm)
HFIP-603 to HFIP-607	(1)5% Acetone (2)2micro-L (3)HFIP (4)0.25mL/min (5)Ambient (6)UV(280nm)
HT-803 to HT-807	(1)10% Monochlorobenzene (2)20micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
UT-802.5 UT-806M UT-807	(1)10% Monochlorobenzene (2)10micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
AT-806MS	(1)1% Acetone (2)20micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
LF-804 LF-604 LF-404	(1)0.1% Propylbenzene(LF-804), 0.1% Dicyclohexyl phthalate(LF-604, LF-404) (2)20micro-L(LF-804), 5micro-L(LF-604, LF-404) (3)THF (4)1.0mL/min(LF-804), 0.5mL/min(LF-604), 0.3mL/min(LF-404) (5)Ambient (6)UV(254nm)