Characteristics and Application of Reversed Phase, Hydrophilic Interaction
and Normal Phase Chromatography Columns
RSpak RP18-415 has a pore size of approximately
450 angstrom and are suitable
for the analysis of proteins. The Asahipak ODP, C8P and C4P have a pore
size of approximately 250 angstrom and are suitable for peptides.
The RSpak DE-613 and DE-413 have a pore size of approximately 100 angstrom can
be used for the analysis of a low molecular weight substances such as oligopeptides.
Should none the DE-613, 413 or ODS columns give satisfactory results due the non-retention
of the sample then the use of the RSpak RP18-413 or the RP18-613 which have a
lower polarity can be recommended.