Features of Multimode Columns (Peptide Analysis)

Although Asahipak GS-HQ series is basically SEC columns, they permit different types of separation and analysis from conventional ones by use of a multimode which combines the hydrophobic interaction (RPC) and ionic interaction (IEX: ion exclusion). The GS-320 HQ column performs well in separating hydrophillic peptides, particularly acidic and basic peptides. As the columns can be used over a wide range of eluent pH because of being polymer-based column, they can be washed with alkaline solution leading to a longer column life, which is another feature of the column.


Sample : 20micro-L
1. Glu-Ala-Glu 0.025%
2. Arg-Asp 0.05%
3. Gly-His-Lys 0.025%
4. Arg-Pro-Lys-Pro 0.025%

	MW	pI	Σf
Glu-Ala-Glu	347	3.12	0.39
Arg-Asp	289	6.75	0.68
Gly-His-Lys	340	9.95	0.29
Arg-Pro-Lys-Pro	497	11.44	3.24

pI : Isoelectric point
Σf : Hydrophobic parameter

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 50mM Ammonium acetate buffer(pH6.7)
Flow rate    : 0.5mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C