Avidin (2)

Avidin, one of the saccaride-binding proteins, was analyzed using aqueous solvent SEC (GFC) column, PROTEIN KW-804.


Sample : 50micro-L
1. Avidin (Wako Pure Chemical Industries, Ltd.) 0.1%

Column     　: Shodex PROTEIN KW-804 (8.0mmID*300mm)
Eluent       : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 1.0mL/min
Detector　   : UV(254nm) 
Column temp. : 30deg-C