Standard Proteins (3) (RP18-415)

RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.

Recovery of Proteins
1. Ribonuclease A	93%
2. Insulin	98%
3. Cytochrome c	100%
4. Lysozyme	100%
5. BSA	98%
6. Myoglobin	108%
7. Ovalbumin	---

Column       : Shodex RSpak RP18-415 (4.6mmID*150mm)
Eluent       : (A); 0.1% TFA aq./CH3CN=99/1
               (B); 0.1% TFA aq./CH3CN=5/95
               Linear gradient: 0 to 25min, 20% (B) to 60% (B)
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : Room temp.