Opioid Peptides (2) (By Pre-column Reaction Method)

Opioid peptides were analyzed using Asahipak ODP-50 6D by pre-column reaction method.


Sample :
1. Tyr-Gly-Gly-Phe
2. ME, Met-Enkephalin
3. LE, Leu-Enkephalin
4. LE-Arg
5. alpha-EDP, alpha-Endorphin
6. LE-NH₂
7. DNP 1-7
8. [D-Ala²] LE-NH₂
9. ME-Arg-Gly-Leu
10. gamma-EDP
11. ME-Arg-Phe
12. DNP 1-8
13. beta-NED, beta-Neoendorphin
14. alpha-NED, beta-NED-Lys
15. DNP 1-9
16. DNP 1-10
17. DNP-B

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : (A); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=10/39/1
               (B); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=2/3/5
               Gradient:(A) to (B)
Flow rate    : (0 to 25min); 0.8mL/min, (25 to 55min); 1.0mL/min, (after 55min); 0.8mL/min 
Detector     : Fluorescence(Ex. 330nm, Em. 430nm)
Column temp. : Room temp.

Guo-Qing Zhang et al., Chem. Pharm. Bull., 39 (1991) 126.