Column : Shodex RSpak DE-613 (6.0mmID*150mm) Eluent : (A); 0.05% TFA aq./CH3CN=96/4 (B); 0.05% TFA aq./CH3CN=75/25 Linear gradient: 0 to 30min, (A) to (B) Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : 30deg-C