Comparison of Peak Height of GS-320 HQ with Semi-micro Columns

In the analysis of uracil with the conventional typed LC systems having injector, detector and tubes designed for the down sized analysis, conventional typed column GS-320 HQ was compared with its semi-micro typed columns. The peak height of uracil analyzed with semi-micro GS320A-2E (2.0mmID) column is twelve times than that analyzed with conventional typed GS-320 HQ (7.5mmID). And the peak height of uracil analyzed with semi-micro GS320A-1E (1.0mmID) column is thirty six times than that analyzed with conventional typed GS-320 HQ (7.5mmID). The high sensitivity can be obtained with your LC system and parts designed for down sized chromatography.


Sample : 10ppm each, 1micro-L
Uracil

Columns  : Shodex GS320A-1E (1.0mmID*250mm)
           Shodex GS320A-2E (2.0mmID*250mm)
           Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent   : 10mM HCOONH4 buffer(pH4.0)
Detector : UV(260nm) cell volume; 3micro-L, optical path length; 7mm
Tubing   : 0.1mmID PEEK tube
Injector : Rheodyne 8125