Separation of Anomer

It has been reported that the anomer separation does not take place at high temperature. Therefore, when using SUGAR series (SP0810, SC1011, KS-801, SZ5532 or SC1211), set up the column temperature at 70-80 deg-C.
Please refer to Prevention of Anomer Separation.

Sample : 0.5% each, 10micro-L
Glucose, Mannose, Xylose, Galactose, Arabinose
Column       : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent       : H2O
Flow rate    : 0.7mL/min
Detector     : Shodex RI
Column temp. : 29deg-C, 70deg-C