Separation of Anomer
It has been reported that the anomer separation does not take place at high temperature.
Therefore, when using SUGAR series (SP0810
), set up the column
temperature at 70-80 deg-C.
Please refer to Prevention of Anomer Separation
Sample : 0.5% each, 10micro-L
Column : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent : H2O
Flow rate : 0.7mL/min
Detector : Shodex RI
Column temp. : 29deg-C, 70deg-C