Milk Coffee

When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before the analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure.

(Sample pre-treatment for milk coffee)
1) Put 5g of the sample in a 10mL messflask.
2) Add ultra pure water to make up 10mL solution.
3) Perform ultrafiltration (mw:10,000).
4) Take a part of the sample solution and add the same volume of acetonitrile.
5) Pass through a 0.45micron membrane filter before injecting into HPLC.


Sample : Milk coffee, 20micro-L
1. Fructose
2. Glucose
3. Sucrose
4. Lactose

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : Room temp.(25deg-C)