Calibration Curves for Saccharides (NH2P-50)

Typical saccharides were analyzed using Asahipak NH2P-50 4E and calibaration curves for the saccharides were obtained. Every calibaration curves passes the origin and has good linearity. All the corelation coefficients for the saccharides are greater than 0.999. The calibration curves were obtained from peak heights. They can be also obtaind from the peak area satisfactorily.

Sample : 20micro-L
Correlation Coefficient
(0.2 to 200micro-g)
Glucose 0.9998
Sorbitol 0.9999
Fructose 0.9998
Sucrose 0.9999
Lactose 0.9997
Maltose 0.9998
Xylitol 0.9999
Mannitol 0.9999
Maltitol 0.9997

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 25deg-C