Analysis of Soft Drinks (LC/MS)

Canned soft drink containing coffee and sugar, tea and sugar and orange juice were analyzed using LC/MS. Stable baselines were obtained by using a polymer-based amino column, Asahipak NH2P-50 2D.


Sample : Soft drink
Canned coffee with sugar
1. Sucrose(m/z=378)

Canned tea with sugar
1. Sucrose(m/z=378)

Canned orange juice
1. Fructose(m/z=216)
2. Glucose(m/z=216)

(HPLC)
Instrument   : Agilent 1100
Column       : Shodex Asahipak NH2P-50 2D (2.0mmID*150mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 0.2mL/min
Column temp. : 40deg-C
(Post column)
Reagent      : CH3CN/CHCl3=50/50
Flow rate    : 0.2mL/min
(MS)
Instrument      : Agilent 1100MSD
Mass range      : 100-500(m/z)
Ionization      : APCl
Mode            : SIM(M+Cl)
Polarity        : Negative
Fragmentor      : 20V
Nebulizer       : N2(40psi)
Drying gas      : N2(10L/min, 350deg-C)
Corona current  : 30micro-A
Vaporizer temp. : 400deg-C