Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
Asahipak
NH2P-40 3E is filled with smaller-sized gel, 4 micro-m, and packed in a 3.0 mm internal diameter (I.D.) column housing. The selected ID size allows the column to be used with a conventional LC system, i.e., without a need of semi-micro or any other special LC system. NH2P-40 3E provides higher resolution compared to Asahipak
NH2P-50 4E. NH2P series is one of the best fits for the saccharides analysis using HILIC mode. The solvent required for NH2P-40 3E column is approximately 1/3 of the NH2P-50 4E column.
Sample : 0.5% each, 4micro-L
1.
Fructose
2.
Glucose
3.
Sucrose
Data obtained by a conventional RI (0.25 mm ID tubing).
| |
NH2P-40 3E |
NH2P-50 4E |
| TPN (Sucrose) |
11,300 |
8,700 |
| Rs |
Fructose/Glucose |
6.4 |
4.2 |
| Glucose/Sucrose |
8.5 |
5.9 |
Columns : Shodex Asahipak NH2P-40 3E (3.0mmID*250mm), NH2P-50 4E (4.6mmID*250mm)
Eluent : H2O/CH3CN=25/75
Flow rate : (NH2P-40 3E) 0.35mL/min, (NH2P-50 4E) 1.0mL/min
Detector : Shodex RI
Column temp. : 25deg-C