Saccharides in Wood (2)

Two sugar analysis columns, SUGAR SP0810, was used to analyze saccharides in wood. Corona charged aerosol detector (CAD) was used as a detector. Comparing to Sacchrides in Wood (1) , the separation of arabinose and mannose was improved by adding CH₃CN to the mobile phase.


Sample : 10micro-L
1. Cellobiose 1.0%
2. Glucose 1.5%
3. Xylose 0.5%
4. Galactose 0.5%
5. Arabinose 0.5%
6. Mannose 1.0%

Column       : Shodex SUGAR SP0810 (8.0mmID*300mm) x 2
Eluent       : H2O/CH3CN=77/13
Flow rate    : 0.6mL/min
Detector     : Corona charged aerosol
Column temp. : 85deg-C