Saccharides and Amino Acids (1)

Generally, ligand exchange mode columns are used with aqueous eluent, but the addition of the same cation as the counter ion enables the columns to separate samples under ion exchange mode additionally to the ligand exchange mode. CaSO4 aqueous solution is used for SUGAR SC1011 as its counter ion is Ca2+. This means that ligand exchange occurs between the counter ions of the packing material and the hydroxyl groups of saccharides and ion exchange occurs between the sulfonate groups and the amino groups of the saccharides. For sensitive measurement, attention should be paid to the positions of minus peak due to the cations in the eluent.

Sample :
1. Maltose
2. Glucose
3. Fructose
4. Glutamic acid
5. Aspartic acid
6. Threonine
7. Serine
8. Glutamine
9. Alanine

Column       : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent       : 0.01M CaSO4(pH6.0)
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80deg-C