Analysis of Mannitol according to USP Method (MN-431)
According to USP method (United States Pharmacopeia 23), mannitol should be
analyzed using a column which can separate mannitol and and sorbitol with R (resolution)
of equal to or greater than 2.0. USPpak MN-431
is a column
specially designed for mannitol analysis, whcih satisfies this criterion.
Column : Shodex USPpak MN-431 (4.0mmID*250mm)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 60deg-C
The measurement of mannitol by USP method should be done at 30 to 85 deg-C within 2 deg-C.