Saccharides and Sugar Alcohols (7) (LC/MS)

Saccharides and sugar alcohols were analyzed using a polymer-based amino column, Asahipak NH2P-50 and LC/MS.


Sample :
1. Sorbitol (m/z=218)
2. Glucose (m/z=216)

Sample :
1. Xylitol (m/z=188)
2. Fructose (m/z=216)
3. Glucose (m/z=216)
4. Sucrose (m/z=378)

(HPLC)
Instrument   : Agilent 1100
Column       : Shodex Asahipak NH2P-50 2D (2.0mmID*150mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 0.2mL/min
Column temp. : 40deg-C
(Post column)
Reagent      : CH3CN/CHCl3=50/50
Flow rate    : 0.2mL/min

(MS)
Instrument      : Agilent 1100MSD
Mass range      : 100-500(m/z)
Ionization      : APCl
Mode            : SIM(M + Cl)
Polarity        : Negative
Fragmentor      : 20V
Nebulizer       : N2(40psi)
Drying gas      : N2(10L/min, 350deg-C)
Corona current  : 30micro-A
Vaporizer temp. : 400deg-C