Apple Juice (SC1011)

Saccharides and a sugar alcohol contained in apple juice were analyzed using SUGAR SC1011 (a column for saccharides analysis).

(Sample pretreatment)
1) Take 5g of apple juice in a beaker, add 30mL of pure water and neutralize with 10%(W/V) NaOH aq. cooled with ice.
2) Apply ultrasonic vivration for 30 minutes and make 50mL solution with pure water.
3) Filtrate with 0.45 micron membrane filter.

Sample : Apple juice 5micro-L
1. Sucrose
2. Glucose
3. Fructose
4. Sorbitol

Column       : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent       : H2O
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 85deg-C