Guidelines for Shodex Column Selection : Drugs, Metabolites and Chiral Compounds
(Drugs and metabolites)
For the analysis of drugs and metabolites, reversed phase mode, ion exchange mode
and multimode can be used. For such analysis, polymer-based columns such as the
Asahipak ODP, RSpak DE, DS and RP18 series are well- suited because they can be
used even with a strong alkaline eluent. For the analysis of basic drugs such
as small amines, the above polymer- based columns are especially suited because
these columns can be used with an eluent of pH10-11. At such conditions, they
has almost the same result as analysis with an ion pair reagent yet avoids the
problems caused by the use of the latter.
Shodex columns are available in three modes for chiral separation. ORpak CD-HQ
columns are packed with polymer-based gel bonded with cyclodextrin derivatives
as ligands. The separation mode is based on the difference in the degree of inclusion
of the samples into the cyclic structure of the ligand. Therefore, these columns
can be used for the separation of position and geometrical isomers as well as
optical ones. The ORpak CRX-894 column is packed with a polymer-based gel to which
L-amino acid derivative is bonded. The AFpak ABA-894, an affinity column packed
with polymer-based gel to which bovine serum albumin (BSA) is bonded as the ligand.
Both columns are suitable for chiral separation of amino acids.