Diuretics (2)
Amiloride was analyzed using RSpak 
DE-613 (a column for reversed phase chromatography).

Sample : 
Amiloride
Column       : Shodex RSpak DE-613 (6.0mmID*150mm)
Eluent       : 1% NH4OH aq./CH3CN=20/80
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : Room temp.