Diuretics (2)
Amiloride was analyzed using RSpak
DE-613 (a column for reversed phase chromatography).
Sample :
Amiloride
Column : Shodex RSpak DE-613 (6.0mmID*150mm)
Eluent : 1% NH4OH aq./CH3CN=20/80
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : Room temp.