Effect of pH of Eluent on Sensitivity of Barbital

Effect of eluent pH on sensitivity of barbital analysis using Asahipak ODP-50 4D (a column for reversed phase chromatography) is shown.The analysis with alkaline eluent(pH 11.0) gives the best result.


Sample : Barbital

Column       : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent       : 25mM Sodium phosphate buffer/CH3CN=60/40
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 30deg-C