Berberine in Japanese Goldthread

Berberine in japanese goldthread were analyzed using Asahipak ODP-50 4D (a column for reversed phase chromatography).


Sample : Berberine in japanese goldthread

Column       : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent       : TFA/H2O/CH3CN=0.05/70/30
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 35deg-C