Sulfanilamides

Sulfanilamides were analyzed using RSpak DE-413 (a column for reversed phase chromatography).


Sample : 20mg/L each, 10micro-L
1. Succinylsulfathiozole
2. Sulfadiazine
3. Sulfamerazine
4. Sulfathiazole
5. Sulfamethazine
6. Sulfanilamide

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : 25mM Sodium phosphate buffer(pH7.6)/CH3CN/=82/18
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 40deg-C