Theophylline in Serum

With Asahipak GS-320 7G, a multimode column, it is possible to make the direct analysis of drug components in serum and urine without sample pretreatment. Proteins, nucleic acids and polysaccharides are eluted first by GFC mode, and then, drugs and metabolites, whose hydrophobic interaction is larger, are eluted by reversed phase mode. This method can be used for the analysis of extracted fluid from serum, urine and internal organs.
GS-320 7G is an old type of GS-320 HQ.


Sample : Serum
1. Protein
2. Unknown
3. Theophylline

Column       : Shodex Asahipak GS-320 7G (7.5mmID*500mm)
Eluent       : 0.01M Sodium acetate (pH4.0)/CH3CN=10/1
Flow rate    : 2.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.

Courtesy of Dr. Nimura, Kitazato University