Hexobarbital in Serum

With MSpak GF-310 4B, a column for LC/MS, it is possible to make the direct analysis of drug components in serum without sample pretreatment.



Sample : Hexobarbital

(HPLC)
Instrument   : Agilent 1100
Column       : Shodex MSpak GF-310 4B (4.6mmID*50mm)
Eluent       : 50mM CH3COONH4/CH3CN=80/20
Flow rate    : 0.5mL/min
Detector     : UV(220nm)
Column temp. : 35deg-C

(MS)
Instrument   : Agilent 1100MSD
Mass range   : 105-500(m/z)
Ionization   : Electrospray
Nebulizer    : N2(50psi)
Drying gas   : N2(12L/min, 350deg-C)
Mode         : Positive SIM (m/z=281)