Reproducibility in LC/MS Analysis (ODP2 HP)

Reproducibility for drug analysis in protein (BSA) was confirmed using polymer-based ODP2 HP. The separation remains good even after 150 runs.


Sample : 500ng/mL each in 0.7mg/mL BSA, 5micro-L
1. Lidocaine
2. Imipramine

Column       : Shodex ODP2 HP-2B (2.0mmID*50mm)
Eluent       : 10mM Ammonium acetate aq./CH3CN=70/30
Flow rate    : 0.2mL/min
Detector     : ESI-MS (TIC positive)
               m/z 235; Lidocaine
               m/z 281; Imipramine
Column temp. : 40deg-C