HVA and VMA Standards

Homovanilic acid (HVA) and Vanillylmandelic acid (MVA) are analyzed using Asahipak ES-502N 7C (a column for weak cation exchange chromatography) by UV detection.


Sample : 100micro-L
HVA and VMA standards
Column       : Shodex Asahipak ES-502N 7C (7.5mmID*100mm)
Eluent       : 50mM Bis-Tris propane(pH7.0 adjusted by HCl) + 200mM NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C