Chlorthalidone (1)

Optical rotation of each peak can be monitored by using a combination of an optical rotation detector (ORD) and ORpak CDB-453 HQ (a column for chiral separation). A positive peak shows dextrorotation, while a negative peak shows levorotation. The results of the analysis of chlorthalidone, a diuretic, using a UV detector and an optical rotation detector is shown.


Sample : Chlorthalidone

Column       : Shodex ORpak CDB-453 HQ (4.6mmID*150mm)
Eluent       : (1.1% CH3COOH + 0.2M NaCl)/CH3CN=90/10
Flow rate    : 0.2mL/min
Detector     : UV(300nm), ORD
Column temp. : 10deg-C