Hydroxyriboflavin and Riboflavin in Urine

For the analysis of vitamin B₂, reversed phase columns are usually used with fluorescence detectors. This shows the analyses of vitamin B₂ by reversed phase mode using Asahipak GS-320 7E, a multimode column.
GS-320 7E is an old type of GS-320 HQ.


Sample :
1. 7alpha-Hydroxyriboflavin
2. Riboflavin (Vitamin B₂)

Column       : Shodex Asahipak GS-320 7E (7.5mmID*250mm)
Eluent       : 1M Propionic acid buffer(pH4.4)
Flow rate    : 1.5mL/min
Detector     : Fluorescence detector(Ex.250nm, Em.520nm)
Column temp. : 55deg-C