Fat-Soluble Vitamins (1) (ODP-50 4E)

Asahipak ODP-50 4E was used for the simultaneous analysis of fat-soluble vitamins. Good calibration curves were obtained for them.


Sample : 20micro-L
1. Vitamin K₃ 1.5micro-g/mL
2. Vitamin A 1.0IU/mL(0.3micro-g/mL)
3. Vitamin A acetate 0.5IU/mL(1.9micro-g/mL)
4. Vitamin D₂ 13.2IU/mL(0.3micro-g/mL)
5. Vitamin D₃ 13.2IU/mL(0.3micro-g/mL)
6. Vitamin E acetate 2.4micro-g/mL
7. Vitamin E 2.5micro-g/mL
8. Vitamin K₁ 2.4micro-g/mL

Column       : Shodex Asahipak ODP-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/CH3OH=50/50
Flow rate    : 0.6mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C