Nutrient Beverage

Nutrient beverage was analyzed using RSpak DE-413 ( a column for reversed phase chromatography ).


Concentration
Contents in 4g
1. Niacinamide
16.5micr-og/mL
24.7mg
2. Vitamin B1
9.4micro-g/mL
14.1mg
3. Vitamin B2
2.9micro-g/mL
4.3mg


Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
               (B); H2O/CH3CN=50/50
               gradient: 0min to 4min, 100% (A), 
                         4min to 15 min, 100% (A) to 30% (A), 
                         After 15 min, 30% (A) 
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 35deg-C