Analysis of Vitamins = Nutrient Beverage

Nutrient Beverage

Nutrient beverage was analyzed using RSpak DE-413 ( a column for reversed phase chromatography ).

Concentration
Contents in 4g

1. Niacinamide
16.5micr-og/mL

24.7mg

2. Vitamin B₁
9.4micro-g/mL

14.1mg

3. Vitamin B₂
2.9micro-g/mL

4.3mg

	Concentration	Contents in 4g
1. Niacinamide	16.5micr-og/mL	24.7mg
2. Vitamin B₁	9.4micro-g/mL	14.1mg
3. Vitamin B₂	2.9micro-g/mL	4.3mg

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
               (B); H2O/CH3CN=50/50
               gradient: 0min to 4min, 100% (A), 
                         4min to 15 min, 100% (A) to 30% (A), 
                         After 15 min, 30% (A) 
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 35deg-C