Comparison of Analysis of PMMA with Down-sized SEC Columns

The calibration curves for downsized organic SEC (GPC) columns using PMMA are shown. In the case of the analysis for polymer whose MW is 1,000,000 or less, GPC LF-604 or LF-404 is suitable. When it is needed to separate low MW polymer and high MW polymer and low MW polymer is the purpose for the analysis, GPC HFIP-603 is suitable.
Please refer to Comparison of Calibration Curves for Down-sized SEC Columns (HFIP Eluent: PMMA)

Sample : 50micro-L
1. PMMA, Poly(methyl methacrylate) (MW: 1,944,000)
2. PMMA, Poly(methyl methacrylate) (MW: 281,700)
3. PMMA, Poly(methyl methacrylate) (MW: 79,250)
4. PMMA, Poly(methyl methacrylate) (MW: 13,300)
5. PMMA, Poly(methyl methacrylate) (MW: 1,960)
Columns      : Shodex GPC LF-604 (6.0mmID*150mm) x 2
               Shodex GPC LF-404 (4.6mmID*250mm) x 2
               Shodex GPC KF-405LHQ (4.6mmID*250mm) x 2
               Shodex GPC KF-406LHQ (4.6mmID*250mm) x 2
               Shodex GPC HFIP-603 (6.0mmID*150mm) x 2
               Shodex GPC HFIP-606M (6.0mmID*150mm) x 2    
Eluent       : 5mM CF3COONa in HFIP
Flow rate    : 0.3mL/min
Detector     : Shodex RI(small cell volume)
Column temp. : 40deg-C